HAVSMC人主動(dòng)脈血管平滑肌
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人主動(dòng)脈血管平滑肌細(xì)胞 HA-VSMC
種屬 | 人 |
別稱 | T/G HA VSMC; T/G HA-vSMC; T/GHA-VSMC; TGHAVSMC; T/G Human Aortic Vascular Smooth Muscle Cells |
組織來源 | 主動(dòng)脈;平滑肌 |
傳代比例/細(xì)胞消化 | 1:2傳代,消化2-3分鐘 |
培養(yǎng)基配置 | The base medium for this cell line is ATCC-formulated F-12K Medium , Catalog No. 30-2004. To make the complete growth medium , add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM , TES to a final concentration of 10 mM. |
形態(tài) | 成纖維細(xì)胞樣 |
生長(zhǎng)特征 | 貼壁生長(zhǎng) |
倍增時(shí)間 | 每周 2 至 3 次 |
STR | Amelogenin:X ,Y;CSF1PO:10 ,11;D13S317:12 ,14;D16S539:10 ,11 ,13;D18S51:12; D19S433:13;D21S11:30 ,33;D2S1338:15 ,16;D3S1358:16;D5S818:10 ,13;D7S820:7, 9 ,11;D8S1179:13 ,14;FGA:22;TH01:7;TPOX:11;vWA:17 ,18; |
培養(yǎng)條件 | 氣相:空氣 ,95% ;二氧化碳 ,5%。 溫度:37攝氏度 ,培養(yǎng)箱濕度為70%-80%。 |
凍存條件 | 凍存液:90%FBS ,DMSO 10%, 或使用非程序凍存液:貨號(hào)JY-H040 |
保藏機(jī)構(gòu) | ATCC; CRL-1999 |
產(chǎn)品使用 | 于科學(xué)研究 ,不可作為動(dòng)物或人類疾病的治療產(chǎn)品使用。 |
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