IPEC-J2 豬小腸上皮細(xì)胞
- 公司名稱 上海富雨生物科技有限公司
- 品牌
- 型號
- 產(chǎn)地
- 廠商性質(zhì) 生產(chǎn)廠家
- 更新時間 2025/2/12 8:47:00
- 訪問次數(shù) 7
聯(lián)系方式:陳壽彬18956075901 查看聯(lián)系方式
聯(lián)系我們時請說明是化工儀器網(wǎng)上看到的信息,謝謝!
產(chǎn)品名稱:IPEC-J2 豬小腸上皮細(xì)胞、IPEC-J2 豬小腸上皮細(xì)胞、IPEC-J2 豬小腸上皮細(xì)胞、IPEC-J2 豬小腸上皮細(xì)胞;
豬正常腸上皮細(xì)胞IPEC-J2
種屬 | 豬 |
別稱 | IPECJ2; Intestinal Porcine Epithelial Cell line-J2 |
組織來源 | 腸(空腸) |
疾病 | 正常 |
傳代比例/細(xì)胞消化 | 1:2傳代 ,消化1-3分鐘 |
培養(yǎng)基配置 | DMEM培養(yǎng)基;10%胎牛血清;1%雙抗 |
簡介 | 該細(xì)胞從一只新生的未感染豬空腸分離的正常腸上皮細(xì)胞中建立 ,經(jīng)歷一個自發(fā)的分化過程 ,在1-2周內(nèi)形成具有低或 高跨上皮電阻(TEER)的極化單層 ,這取決于加入培養(yǎng)基的血清類型。 |
形態(tài) | 上皮細(xì)胞樣 |
生長特征 | 貼壁生長 |
倍增時間 | 每周 2 至 3 次 |
培養(yǎng)條件 | 氣相:空氣 ,95% ;二氧化碳 ,5%。 溫度:37攝氏度 ,培養(yǎng)箱濕度為70%-80%。 |
凍存條件 | 凍存液:90%FBS ,DMSO 10%, 或使用非程序凍存液:貨號JY-H040 |
保藏機(jī)構(gòu) | DSMZ; ACC-701 |
產(chǎn)品使用 | 于科學(xué)研究 ,不可作為動物或人類疾病的治療產(chǎn)品使用。 |
The aim of this study is to determine whether the pretreatment CD8+PD-1+ to CD4+PD-1+ (PERLS) ratio is an independent risk prognostic factor of advanced melanoma patients. We retrospectively analyzed the efficacy and flow cytometry data from advanced melanoma patients who received PD-1 inhibitor as monotherapy between January 1, 2018 and January 26, 2022. Fifty-nine patients were enrolled, the PERLS cutoff was 1.125. PERLS did not correlate with clinical characteristics but were significantly associated with baseline CD8+, CD4+, and CD8+PD-1+ T cells. The mean overall survival and the progression-free survival were 45.8 and 17.1?months for the low PERLS group (n?=?39), compared with 29.9 (P?=?0.001) and 9.7 (P?=?0.003) months for the high PERLS group (n?=?20), respectively. Pretreatment PERLS might contribute to selecting patients before receiving anti-PD-1 therapy.