HCC827人非小細(xì)胞肺癌細(xì)胞

細(xì)胞名稱

HCC827人非小細(xì)胞肺癌細(xì)胞

描述

這株細(xì)胞建于1994年三月。這株肺腺癌在EGFR酪氨酸激酶區(qū)域有一個(gè)獲得性突變（E746-A750缺失）。 患者在25歲到26歲時(shí)每個(gè)月抽1包煙。 在診斷前12年不再抽煙。

動物種別

人

性別

女

組織來源

肺腺癌

形態(tài)

上皮細(xì)胞樣

培養(yǎng)基和添加劑

添加２mM L-谷氨酰胺、10mM HEPES、1mM 丙酮酸鈉、4.5克/升葡萄糖、1.5克/升碳酸氫鈉的RPMI 1640，90%； 及： 胎牛血清，10%。 這個(gè)配方用于空氣中充入5%CO2。

REFERENCE

Girard L , et al. Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and non-small cell lung cancer, and loci clustering. Cancer Res. 60: 4894-4906, 2000. PubMed: 10987304 Burbee DG , et al. Epigenetic inactivation of RASSF1A in lung and breast cancers and malignant phenotype suppression. J. Natl. Cancer Inst. 93: 691-699, 2001. PubMed: 11333291 Toyooka S , et al. Differential expression of FEZ1/LZTS1 gene in lung cancers and their cell cultures. Clin. Cancer Res. 8: PubMed: 12114433 Virmani A , et al. Aberrant methylation of the cyclin D2 promoter in primary small cell, nonsmall cell lung and breast cancers. Int. J. Cancer 107: 341-345, 2003. PubMed: 14506731 Liu CX , et al. LRP-DIT, a putative endocytic receptor gene, is frequently inactivated in non-small cell lung cancer cell lines. Cancer Res. 60: 1961-1967, 2000. PubMed: 10766186 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. （1992）, ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., （1995） Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Centers for Disease Control （1993）, Biosafety in Microbiological and Biomedical Laboratories Human Health Service Publication No. （CDC） 93-8395. U.S. Dept. of Health and Human Services; 3rd Edition U.S. Government Printing Office Washington D.C.