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郵編:102208
聯系人:付經理
電話:010-52571502
傳真:QQ:2459729122
手機:15910725349
留言:發(fā)送留言
網址:www.hedebio.com
商鋪:http://m.true-witness.com/st121773/
資料下載
天狼猩紅染液
最近更新時間:2012-11-22
提 供 商:海德創(chuàng)業(yè)(北京)生物科技有限公司資料大?。?/span>61.6KB
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詳細介紹:
0.1%的天狼猩紅溶于飽和苦味酸溶液
海德創(chuàng)業(yè)(北京)生物科技有限公司
This method eliminates cytoplasmic staining, revealing thin septa and collagen fibers clearly.
Fixation
Bouins' Fixative
Sections
Parrafin @ 7 microns.
Staining
1. Deparaffinize and hydrate to distilled water.
2. Wash in running tap water, 10 minutes.
3. Rinse in distilled water.
4. Treat in Phosphomolybdic Acid 0.2% Aqueous (1-5 minutes)
5. Stain in Sirius Red, 0.1% in Saturated Picric Acid , for 90 minutes.
6. Wash for 2 minutes in 0.01 N Hydrochloric Acid .
7. Rinse in 70% Alcohol for 45 seconds.
8. Dehydrate, clear and mount in Permount
Results
Septa | Red |