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Flt3 Ligand Human, Sf9
  • Flt3 Ligand Human, Sf9
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貨物所在地：福建廈門市

更新時間：2024-11-24 21:00:06

瀏覽次數(shù)：110

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Flt3 Ligand Human, Sf9
產(chǎn)品編號：CYT-409
產(chǎn)品規(guī)格：2μg/10μg/0.1mg

Synonyms Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
Introduction FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation.
Description Flt3-Ligand Human Recombinant produced in Sf9 insect cells is a glycosylated, polypeptide chain containing 155 amino acids and having migrating on SDS-PAGE at 19kDa.
Flt3-Ligand is purified by proprietary chromatographic techniques.
Source Baculovirus Sf9 cells.
Physical Appearance Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation The protein was lyophilized with no additives.
Solubility It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
Stability Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-L should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein （0.1% HSA or BSA）.
Please prevent freeze-thaw cycles.
Purity Greater than 95.0% as determined by（a） Analysis by RP-HPLC.
（b） Analysis by SDS-PAGE.
Amino acid sequence The sequence of the first five N-terminal amino acids was determined and was found to be Thr-Gln-Asp-Cys-Ser.
Biological Activity The ED50, calculated by the dose-dependant stimulation of the proliferation of human MUTZ-2cells is < 3.0 ng/ml.
Protein content Protein quantitation was carried out by two independent methods
1. UV spectroscopy at 280 nm using the absorbency value of 1.42 as the extinction coefficient for a 0.1% （1mg/ml） solution. This value is calculated by the PC GENE computer analysis program of protein sequences （InliGenetics）.

2. Analysis by RP-HPLC, using a standard of Flt3-Ligand as a Reference Standard.
Usage ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

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