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當(dāng)前位置:上海一基實(shí)業(yè)有限公司>>生物試劑>>酶類>> M0064Super Script Ⅱ陰性逆轉(zhuǎn)錄酶
產(chǎn)品型號(hào)M0064
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廠商性質(zhì)經(jīng)銷商
所 在 地上海市
更新時(shí)間:2025-01-20 14:10:49瀏覽次數(shù):717次
聯(lián)系我時(shí),請(qǐng)告知來自 化工儀器網(wǎng)M0064 | Super Script Ⅱ陰性逆轉(zhuǎn)錄酶/SuperScript II Reverse Transcriptase | BR,200u/ul, | 2000U | |
10000U |
英文名稱:SuperScript II Reverse Transcriptase
級(jí)別:BR
來源:Purified from E. coli expressing the pol gene of M-MLV (1,2), mutagenized to reduce the RNase H activity
濃度:200u/ul
活力定義:One unit of SuperScript® II is the amount of enzyme required to incorporate 1 nmole of eoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)•oligo(dT)12-18 as template•primer (3).
Unit Reaction Conditions:50 mM Tris-HC1 (pH 8.3), 40 mM KC1, 6 mM MgC1 2 , 1 mM DTT, 0.5 mM [3H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)12-18 , 0.1 mg/m1 BSA, and enzyme in 50 µl for 10 min. at 37℃
Performance and Quality Testing:SDS-PAGE purity;endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; and yield and length of cDNA product
Enzyme Function:RNA dependent DNA Polymerase
Final Product Size:12.3 kb or less
Number of Reactions:10 Reactions
Optimal Reaction Temperature:42℃
Sensitivity:Medium
Template:ssRNA;ssDNA;RNA-DNA Hybrid
產(chǎn)品描述:SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mutations rather than a deletion in the RNase H active center. Like SuperScript® RT, SuperScript® II RT has reduced RNase H activity. Unlike SuperScript® RT, however, the selective mutations within the RNase H domain maintain full polymerase activity. This structural modification eliminates degradation of RNA molecules during first-strand cDNA synthesis and gives SuperScript® II RT superior performance characteristics, including:Greater first-strand cDNA yields;More full-length cDNA synthesis;Full activity at 42℃
性狀:懸浮液
用途:生化研究。
保存:-20℃
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