【文獻標題】Short-chain aldehydes increase aluminum retention and sensitivity by enhancing cell wall polysaccharide contents and pectin demethylation in wheat seedlings

【作者】Xin Liang, Yiqun Ou, Hongcheng Zhao,et.al

【作者單位】浙江大學（Zhejiang University）

【文獻中引用產(chǎn)品】

果膠甲酯酶（PME）ELISA試劑盒 活性

【關(guān)鍵詞】Al toxicity ,Cell wall ,Pectin methylation ,Short-chain aldehyde ,Al accumulation ,Wheat 

【DOI】10.1016/j.jhazmat.2022.128743 

【影響因子(IF)】10.58

【出版期刊】《Journal of Hazardous Materials》

【產(chǎn)品原文引用】

2.12. Detection of PME activity 

Pectin methylesterase activity was measured using an ELISA kit (Shanghai Hengyuan Biological Technology Co., Ltd). Approximately 

0.1 g fresh weight of root tips (0–10 mm) was homogenized in 0.9 mL of PBS (50 mM, pH 7.0), and the extracts were centrifuged at 12,000g at 4 ℃ for 20 min. The crude enzyme extracts were used to determine the activities of pectin methylesterase by following the manufacturer’s instructions. 

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