24-square deepwell plates 24平方深孔板

24-square deepwell plates 24平方深孔板
24平方深孔板（用于文庫相對較小的克隆選擇，以及更詳細地研究所選克?。〤HO細胞的標準條件：?2.5-3.5毫升培養(yǎng)體積?225轉(zhuǎn)/分時50毫米振蕩幅度，或300轉(zhuǎn)/分時25毫米振蕩幅度?三明治蓋CR1224a

24-square deepwell plates 24平方深孔板

24-square deepwell plates 24平方深孔板

24平方深孔板（用于文庫相對較小的克隆選擇，以及更詳細地研究所選克?。〤HO細胞的標準條件：•2.5-3.5毫升培養(yǎng)體積•225轉(zhuǎn)/分時50毫米振蕩幅度，或300轉(zhuǎn)/分時25毫米振蕩幅度•三明治蓋CR1224a，或CR1224c•夾鉗CR1700（能夠快速取出和放回MTPs+封蓋），或•夾鉗CR1800（多用途夾鉗，如果也使用淺孔板，則為理想選擇）更多細節(jié)：使用24平方深孔微型板培養(yǎng)CHO細胞比使用96平方深孔板（如上所述）重要得多。50毫米/225轉(zhuǎn)/分和25毫米/300轉(zhuǎn)/分對2.5至3.5毫升的培養(yǎng)量都有很好的效果。據(jù)報道，振幅為12毫米的搖瓶器也有很好的效果（如果以大約500轉(zhuǎn)/分的速度運行）。振幅為3毫米的搖瓶器不能用于24平方深的平板：培養(yǎng)液不能跟隨搖瓶器的運動，因此會出現(xiàn)所謂的異相條件（見右圖），因為培養(yǎng)體積較大，結(jié)果通常是可重復的，當然也包括細胞的數(shù)量它們的產(chǎn)物（如抗體）更大。因此，這種平板格式通常用于對初選擇的96孔平板格式的克隆進行更詳細的研究。如果每隔幾天培養(yǎng)一次，使用大2.5毫升培養(yǎng)體積。在較大的培養(yǎng)體積下，培養(yǎng)物離蓋子太近（甚至可能與蓋子直接接觸），并且在喂食過程中（在取下三明治蓋子，并再次將其放回原處）更容易發(fā)生交叉污染。

 24-square deepwell plates  (for clone selection with relatively small libraries, and for studying selected clones iin more detail)Standard conditions for CHO-cells:•2.5-3.5 ml culture volumes •50 mm shaking amplitude at 225 rpm, or •25 mm shaking amplitude at 300 rpm •sandwich covers CR1224a, or CR1224c •clamps CR1700 (enabling quickly taking out and putting back MTPs + covers), or •clamp CR1800 (versatile clamp, ideal if also shallow well plates are used)Further details: The use of 24-square deepwell microplates for the c*tion of CHO-cells is much less critical than the use of 96-square deepwell plates as described above. Both 50 mm / 225 rpm, and 25 mm / 300 rpm give good results for culture volumes between 2.5 and 3.5 ml. Also shakers with an amplitude of 12 mm have been reported to give good results (if run at approx 500 rpm). Shakers with an amplitude of 3 mm - however - can not be used for 24 square deepwell plates: the culture fluid can not follow the movement of the shaker, and so called out-of-phase conditions will occur (see photo on the right)Because of the higher culture volume, the results are generally well reproducible, and of course also the amounts of cells and their products (such as antibodies) are larger. Therefore, this plate format is often used for more detailed studies on clones that were initially selected in the 96-well plate format. If the cultures are to be fed every few days, it is advisable to use maximally 2.5 ml culture volume. At larger culture volumes, the culture is too close to the cover (and may even get in direct touch with the cover), and cross-contamination is more likely to occur during the feeding procedure (during taking of the sandwich cover, and putting it back on again).

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