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EpiQuik核蛋白提取試劑盒I
EpiQuik Nuclear Extraction Kit *
100 assays/2002元 （貨號:OP-0002-100）
品牌：美國epigentek
Product Overview
The EpiQuik™ Nuclear Extraction Kit provides a simple and selective method along with all necessary reagents for extracting nuclear proteins used for a variety of applications in just 60 minutes. These applications may include western blotting, protein-DNA binding assays, nuclear enzyme assays, and any other procedures requiring optimized nuclear proteins. The protocol is fast and easy-to-use, and also isolates very abundant yields of nuclear extract from mammalian cells or tissue samples.
The EpiQuik™ Nuclear Extraction Kit is also specifically designed to meet the requirements of nuclear extracts used in various EpiQuik™ assays.
The EpiQuik™ Nuclear Extraction Kit simply applies our proprietary nuclear protein isolation buffers to cell/tissues. After treatment with pre-lysis and lysis buffers, the nuclear proteins are easily extracted for immediate use or storage at proper conditions.
To demonstrate the quality of the materials and protocol of the Nuclear Extraction Kit, nuclear extracts were prepared from MCF-7 cells and the activity of HDACs were measured using different amounts of the extract. The result shown in the figure demonstrates the kit's high specificity.
Product Components
NE1 （10X Pre-Extraction Buffer）
NE2 （Extraction Buffer）
1000X DTT Solution
1000X Protease Inhibitor Cocktail （PIC）
User Guide
Frequently Asked Q's
1. Should DTT be added into NE1 for tissue extraction?
It is not necessary.
2. How many rpm should be used for centrifuging the cell extract if using desk-top centrifuge?
12,000-14,000 rpm.
3. What should I do if addition of 5 µl NE2 into 1 M of cells can not suspend the nuclei?
Add more NE2, Ex: 10 µl to 1 M nuclei.
4. Can more solution be added for assay reaction if more nuclear extracts will be added?
Yes.
User Guide & MSDS
 | [User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name. |
| [Material Safety Data Sheet] |
Product Citations
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