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  • Epigentek,P-2014,P-2014-24,P-2014-48,*,植物免疫沉淀(ChIP)試劑盒,EpiQuik Plant ChIP Kit

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貨物所在地: 北京北京市
產(chǎn)地: 美國(guó)epigentek
更新時(shí)間: 2024-09-21 21:00:07
期: 2024年9月21日--2025年3月21日
已獲點(diǎn)擊: 1936
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產(chǎn)品簡(jiǎn)介

中文名 植物免疫沉淀(ChIP)試劑盒
英文名:EpiQuik Plant ChIP Kit

價(jià)格/規(guī)格:24次/4046元(貨號(hào): P-2014-24);48次/6566元(貨號(hào): P-2014-48)

美國(guó)epigentek

Product Overview

詳細(xì)介紹

中文名 植物免疫沉淀(ChIP)試劑盒
英文名:EpiQuik Plant ChIP Kit 

價(jià)格/規(guī)格:24次/4046元(貨號(hào): P-2014-24);48次/6566元(貨號(hào): P-2014-48)

美國(guó)epigentek

景資料 EpiQuik 植物染色質(zhì)免疫共沉淀(ChIP)試劑盒可以有效進(jìn)行體外檢測(cè)植物中蛋白質(zhì)-DNA相互作用,操作方便。操作過(guò)程不到六小時(shí)就可以獲得比其它試劑盒更好的結(jié)果。試劑盒獲得的染色質(zhì)可用來(lái)進(jìn)行定性和定量PCR、southern 印跡以及DNA微陣列分析。
產(chǎn)品描述 EpiQuik 植物染色質(zhì)免疫共沉淀(ChIP)試劑盒提供了對(duì)植物細(xì)胞樣品進(jìn)行染色質(zhì)免疫沉淀反應(yīng)所需的所有試劑。并且,試劑盒中還有一種ChIP級(jí)二甲基組蛋白H3-K9抗體和一種陰性對(duì)照抗體(正常小鼠的IgG)。從細(xì)胞樣品中提取出來(lái)的染色質(zhì)進(jìn)行適當(dāng)?shù)拇驍?,然后加入到微孔中與其表面上吸附的抗體發(fā)生免疫反應(yīng)。特異性結(jié)合到微孔上的DNA從抗體-捕獲蛋白-DNA復(fù)合物上釋放出來(lái),翻轉(zhuǎn)后,通過(guò)本公司專(zhuān)門(mén)設(shè)計(jì)的高速離心柱純化。洗脫下來(lái)的DNA可用于隨后的各種分析。該植物染色質(zhì)免疫沉淀(ChIP)試劑盒 內(nèi)主要產(chǎn)品組份包括:CP1 (Wash Buffer) CP2 (Antibody Buffer) CP3C (5X Lysis Buffer I) CP3D (Lysis Buffer II) CP3E (Lysis Buffer III) CP3F (Lysis Buffer IV) CP4 (ChIP Dilution Buffer) CP5 (DNA Release Buffer) CP6 (Reverse Buffer) CP7 (Binding Buffer) CP8 (Elution Buffer) Protease Inhibitor Cocktail (100X) Normal Mouse IgG (1 mg/ml) Anti-Dimethyl H3-K9 (1 mg/ml) Proteinase K (10 mg/ml) 8-Well Assay Strips (with frame) 8-Well Strip Caps F-Spin Column F-Collection Tube用戶(hù)指南手冊(cè)
產(chǎn)品特點(diǎn) 1、市場(chǎng)上同類(lèi)產(chǎn)品中zui快捷的試劑盒,整個(gè)處理過(guò)程不到6小時(shí);2、96孔板模式使研究人員能根據(jù)自己需要選擇手工或是高通量分析;3、附有DNA純化柱子:大量減少不必要的重復(fù)勞動(dòng);4、操作簡(jiǎn)便、結(jié)果可靠、統(tǒng)一的分析條件;
保存建議 在接收到Epigentek試劑盒后請(qǐng)按照說(shuō)明書(shū)建議,使用不同的保存條件或溫度來(lái)保存試劑盒內(nèi)組分。

Product Overview

The EpiQuik™ Plant ChIP Kit is a convenient package of tools that allows the experimenter to investigate protein-DNA interaction in vivo efficiently. The entire procedure can be completed within 6 hours and produces far superior results than any competitor kits. The EpiQuik™ Plant ChIP kit is suitable for combining the specificity of immunoprecipitation with qualitative and quantitative PCR'' MS-PCR'' DNA sequencing'' and southern blot as well as DNA microarray.

WHY CHOOSE THE EPIQUIK™ PLANT ChIP KIT?
  • The fastest procedure available'' which can be finished within 6 hours.
  • Strip microwell format makes the assay flexible: manual or high throughput.
  • Columns for DNA purification are included: save time and reduce labor.
  • Compatible with all DNA amplification-based approaches.
  • Simple'' reliable'' and consistent assay conditions.
Principle & Procedure


The EpiQuik™ Plant ChIP kit contains all reagents required for carrying out a successful chromatin immunoprecipitation from plant cells. Particularly'' this kit includes a ChIP-grade dimethyl-histone H3-K9 antibody and a negative control normal mouse IgG. Chromatin from the cells is extracted'' sheared'' and added into the microwell immobilized with the antibody. DNA is released from the antibody-captured protein-DNA complex'' reversed and purified through the specifically designed Fast-Spin Column. Eluted DNA can be used for various down-stream applications.

 

SCHEMATIC PROCEDURE:  

 

Product Components

CP1 (wash buffer)
CP2 (antibody buffer)
CP3C (5x lysis buffer I)
CP3D (lysis buffer II)
CP3E (lysis buffer III)
CP3F (lysis buffer IV)
CP4 (CHIP dilution buffer)
CP5 (DNA release buffer)
CP6 (reverse buffer)
CP7 (binding buffer)
CP8 (elution buffer)
Protease inhibitor cocktail (100x)*
Normal mouse IgG (1 mg/ml)*
Anti-dimethyl H3-K9 (1mg/ml)*
Proteinase K (10 mg/ml)*
8-well assay strips (with frame)
8-well strip caps
F-spin column
F-collection tube
User guide

* Spin the solution down to the bottom before use.
User Guide & MSDS

[User Guide]*
*Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name.

[Material Safety Data Sheet]
References & Citations

Shibuya'' Kenichi et al. (Feb 2009). RNA-directed DNA methylation induces transcriptional activation in plants. PNAS 106(5): 1660-5. PubMed Abstract

Barrero'' José María et al. (Sep 2007). INCURVATA2 Encodes the Catalytic Subunit of DNA Polymerase {alpha} and Interacts with Genes Involved in Chromatin-Mediated Cellular Memory in Arabidopsis thaliana. Plant Cell 19(9): 2822-38. PubMed Abstract
 

 

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