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世聯(lián)博研（北京）科技有限公司 |
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—— 銷售熱線 ——
13261877206 |
中文名 超敏甲基化DNA定量試劑盒,
英文名：SuperSense Methylated DNA Quantification Kit
價(jià)格/規(guī)格:48次/4172元（貨號(hào): p-1021-48）；
96次/7546元（貨號(hào): p-1021-96）
美國(guó)epigentek
| 景資料 | SuperSence 甲基化DNA定量試劑盒是一套完整實(shí)驗(yàn)組件，通過熒光檢測(cè)少量DNA樣本中甲基化DNA。本試劑盒適用于檢測(cè)來自任何種系基因組中DNA的甲基化狀態(tài)，例如來自種系：哺乳動(dòng)物，植物，真菌，細(xì)菌和病毒；多種形式包括：各種各樣培養(yǎng)細(xì)胞，新鮮或者冰凍組織，石蠟包埋組織，血漿/血清樣本和生物流體樣本。 | ||
| 產(chǎn)品描述 | SuperSence 甲基化DNA定量試劑盒包含定量總體DNA甲基化的所有試劑。微孔板包被高親和力DNA底物用于固定DNA。被甲基化的DNA通過ELISA-like反應(yīng)被5-甲基胞嘧啶抗體所識(shí)別。大量被甲基化的DNA或者甲基化比率與熒光強(qiáng)度成比例，然后通過熒光檢測(cè)法測(cè)定。 | ||
| 產(chǎn)品特點(diǎn) | 1、基于熒光的實(shí)驗(yàn)簡(jiǎn)單易用，整個(gè)流程不超過2小時(shí)30分鐘；2、高靈敏度，甲基化DNAzui低檢測(cè)極限為50pg，DNAzui低上樣量為2ng；3、通用陽性對(duì)照能定量來自任何物種的甲基化DNA；4、微孔板形式讓實(shí)驗(yàn)更靈活：適用于手工操作或者高通量分析。 | ||
| 保存建議 | 在接收到Epigentek試劑盒后請(qǐng)按照說明書建議，使用不同的保存條件或溫度來保存試劑盒內(nèi)組分。 | ||
The SuperSense™ Methylated DNA Quantification Kit is a complete set of essential components which enables the experimenter to fluorometrically measure levels of methylated DNA using a small amount of DNA samples. The SuperSense™ Methylated DNA Quantification Kit is suitable for detecting global DNA methylation status using genomic DNA isolated from any species such as mammal, plant, fungus, bacteria and virus in a variety of forms including cultured cells, fresh and frozen tissues, paraffin-embedded tissues, plasma/serum samples, and body fluid samples, etc.
WHY CHOOSE THE SUPERSENSE™ METHYLATED DNA QUANTIFICATION KIT?
The SuperSense™ Methylated DNA Quantification Kit contains all reagents required for fluorescent quantification of global DNA methylation. In this assay, DNA is immobilized to the strip well specifically coated with a DNA affinity substance. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to fluorescence intensity, can then be fluorometrically quantified.
| SCHEMATIC PROCEDURE: | |
 |   Quantification of methylated DNA using the fluorometric SuperSense Methylated DNA Quantification Kit. |
SA1 （10X Wash Buffer）
SA2 （DNA Binding Solution）
SA3 （Positive Control, 100 µg/ml）**
SA4 （Block Solution）
SA5 （Capture Antibody, 1000 µg/ml）*
SA6 （Labeling Solution）*
SA7 （Fluoro Developer）*
SA8 （Fluoro Enhancer）*
SA9 （Fluoro Dilution）
8-Well Assay Strips （with Frame）
User Guide
* Spin the solution down to the bottom before use.
** This control is synthesized polynucleotide methylated at every 5-cytosine.
Wong, CP. et. al. （September 2011）. Induction of regulatory T cells by green tea polyphenol EGCG. Immunol Lett. 139（1-2）:7-13.PubMed Abstract
Geyer, K. et. al. （August 2011）. Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nat Commun. 2:424. PubMed Abstract
Cho, YH. et. al. （July 2011）. Identification of transcriptional regulatory elements required for the Mup2 expression in circadian clock mutant mice. Biochem Biophys Res Commun. 410（4）:834-40. PubMed Abstract
 | [User Guide]* *Always use the actual User Guide that shipped with your product. Is the above file locked? You can also request user guides by ing info@epigentek.com along with your contact information and institution name. |
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