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MHC, Class II 抗體

抗原
MHC, Class II
別名 Aalpha, H-2Aa, H2Aa, I-Aalpha, IAalpha, Ia-1, Ia1
適用
其他選擇 Chicken
Murine(116),Rat ((Rattus)) (81),Human (79),Dog (Canine) (19),Cat (12),Chicken (10),Sheep (Ovine) (10),Horse (Equine) (5),Bovine (3),Guinea Pig (3),Goat (1)
宿主
其他選擇 Mouse
Mouse(147),Rat (75),Rabbit (36)
克隆類型 (克隆位點)
單克隆 (2G11)
標記
其他選擇 FITC
FITC(46),Biotin (32),PE (25),APC (5),PE,Cy5 (4),Alexa Fluor 350 (3),Alexa Fluor 488 (3),Alexa Fluor 555 (3),Alexa Fluor 647 (3),Allophycocyanin (3),Cy3 (3),Cy5 (3),Cy5.5 (3),Cy7 (3),HRP (3),APC eFluor 780 (2),EFluor 450 (2),Alexa Fluor 700 (1)
應(yīng)用范圍
其他選擇 Flow Cytometry (FACS), Immunohistochemistry (IHC)
Flow Cytometry (FACS)(192),Immunohistochemistry (Frozen Sections) (IHC (fro)) (68),Western Blotting (WB) (50),Immunoprecipitation (IP) (37),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (35),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (30),Functional Studies (Func) (11),ELISA (10),Immunohistochemistry (IHC) (8),Immunofluorescence (IF) (6),Enzyme Immunoassay (EIA) (2),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (2),Transfected cell culture (TCC) (2),Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1)
[Pubmed] 2 references available
規(guī)格 0.5 mg
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MHC, Class II 抗體

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產(chǎn)品細節(jié)

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克隆位點 2G11
亞型 IgG1
產(chǎn)品特性 The antibody detects: Chicken MHC Class II (B-L) molecules
純化方法 The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

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目標詳細情況

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別名 MHC Class II
背景 The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).
研究領(lǐng)域 Immunology, Adaptive Immunity, Major Histocompatibility Complex (MHC)

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應(yīng)用備注 Working Dilution:
FACS: 1 µg/10^6 cells
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
樣本量 1 mL
限制 For Research Use only

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緩沖液 Product is supplied as 0.5 mg in 1.0 mL of PBS / NaN3.
儲存液 Sodium azide
注意事項 WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediay. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
注意事項 Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.
儲存條件 4 °C

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