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上海宸喬生物科技有限公司>>Ultrasep ES液相色譜柱>>Clinical Chemistry(臨床化學(xué))>>Amino Acids and Peptides

Amino Acids and Peptides

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The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSY

詳細(xì)介紹

Amino Acid and Peptide Analysis

 

UltraSep ESD AP

  

The column is suitable for the separation of derivatives of amino acids (A) and peptides (P). The common procedures are summarized in the following table. If samples are not often analyzed, esp. DABSYLchloride shows advantages.

Comparison of different procedures for derivatization

Procedure

Detection

Limit of detection

Remarks

DABS

Absorption
436 nm

< 1 pmol

Stable derivatives; primary and secondary amino acids can be determined

OPA

Fluorescence
330/440 nm

< 1 pmol

Pro, Hypro and Cys cannot be determined 

FMOC

Fluorescence
265/315 nm

< 1 pmol

His and Cys will be detected only in part

DABS = DABSYLchloride (Dimethylaminoazobenzenesulfonylchloride)
OPA = o-Phthalaldehyde
FMOC = 9-Fluorenylmethoxycarbonylchloride

 

Examples of Analyzed Compounds

Analyzed in Application

Go to

 

Alanine (Ala)

AA

 

 

Arginine (Arg)

AA

AA

 

Aspartic acid (Asp)

AA

 

 

Cystine (Cys2)

AA

EPHARM6

 

Glutamic acid (Glu)

AA

 

 

Glycine (Gly)

AA

 

 

Glutathione

EPHARM6

 
 

Histidine (His)

AA

 

 

Isoleucine (Ile)

AA

 

 

Leucine (Leu)

AA

 

 

Lysine (Lys)

AA

 

 

Methionine (Met)

AA

 

 

Ornithine (Orn)

AA

 

 

Phenylalanine (Phe)

AA

 

 

Proline (Pro)

AA

 

 

Serine (Ser)

AA

 

 

Threonine (Thr)

AA

 

 

Tyrosine (Tyr)

AA

 

 

Valine (Val)

AA

 

 

COLUMNS and  PRICES  ()
 Column size (mm)
250 x 4250 x 3250 x 2125 x 4125 x 3125 x 2
UltraSep ESD AP360.00340.00355.00320.00295.00290.00
 

Size (mm)

 
10 x 410 x 310 x 2
Precolumn cartridges
(Price per pack = 5 pieces)
170.00170.00170.00

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