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詳細(xì)介紹

概述
形態(tài)	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 0.02 % Tween-20.
來(lái)源	Escherichia coli.
氨基酸序列	AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF
內(nèi)毒素水平	Less than 0.1 EU/µg of Recombinant HumanEG-VEGF as determined by LAL method.
活性	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using bovine EJG cells is less than 2.0 μg/ml, corresponding to a specific activity of > 500 IU/mg.
Accession #	P58294
預(yù)期分子量	Approximay 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids.

性能
溶解方法	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
保存方法	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
純度	> 98 % by SDS-PAGE and HPLC analyses.
Gene IDs	84432