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北京百奧創(chuàng)新科技有限公司

[供應]a549d-komda5—A549-Dual KO-MDA5 Cells 返回列表頁

貨物所在地：北京北京市

更新時間：2024-03-27 13:43:03

有效期：2024年3月27日 -- 2025年3月28日

已獲點擊：36

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A549-Dual™ KO-MDA5 cells were generated from A549-Dual™ cells through the stable knockout of the MDA5 gene. They are adherent epithelial cells derived from the human A549 lung carcinoma cell line by stable integration of two inducible reporter constructs. The A549 cell line, a cellular model for asthma and respiratory infections, expresses many pattern recognition receptors (PRRs), including RIG-I [1, 2], and the Toll-like receptors (TLRs) TLR2 [3], TLR3 and TLR5 but not TLR4 [3].

A549-Dual™ KO-MDA5 and A549-Dual™ cells express a secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the IFN-β minimal promoter fused to five NF-κB binding sites. They also express the secreted Lucia luciferase reporter gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. As a result, they allow to simultaneously study the NF-kB pathway, by assessing the activity of SEAP, and the interferon regulatory factor (IRF) pathway, by monitoring the activity of Lucia luciferase. Both reporter proteins are readily measurable in the cell culture supernatant when using QUANTI- Blue™ Solution, a SEAP detection reagent, and QUANTI?Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferease detection reagent.

北京百奧創(chuàng)新科技有限公司

北京百奧創(chuàng)新科技有限公司

[供應]a549d-komda5—A549-Dual KO-MDA5 Cells 返回列表頁

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