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[供應(yīng)]RIG-I Knockout - Reporter Human Lung Carcinoma

貨物所在地：北京北京市

更新時(shí)間：2024-03-29 14:02:58

有效期：2024年3月29日 -- 2025年3月30日

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3-7 x 106 A549-Dual™ KO-RIG-I hACE2-TMPRSS2 cells in a cryovial or shipping flask.

Product Name：RIG-I Knockout - Reporter Human Lung Carcinoma

Cat：a549d-korigi-cov2r

Cell Line Stability 

Cells undergo genotypic changes resulting in reduced responsivenessover time in normal cell culture conditions. Genetic instability is abiological phenomenon that occurs in all stably transfected cells.Therefore, it is critical to prepare an adequate number of frozen stocksat early passages. A549-Dual™ KO-RIG-I hACE2-TMPRSS2 cells shouldnot be passaged more than 20 times to remain fully efficient. 

Quality Control 

• The overexpression of the human ACE2 (hACE2) gene has beenverified by RT-qPCR, FACS staining, and functional assays.

• The overexpression of the human TMPRSS2 (hTMPRSS2) gene hasbeen verified by RT-qPCR and functional assays. 

• RIG-I KO has been verified by (RT-q)PCR and functional assays. 

• Lucia luciferase and SEAP reporter activities have been validatedusing functional assays. 

• The stability for 20 passages following thawing has been verified 

• These cells are guaranteed mycoplasma-free.

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