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[供應(yīng)]bb-ifng——Murine B16 melanoma IFN-γ reporter cells

貨物所在地:北京北京市

更新時(shí)間:2024-03-29 14:02:43

有效期:2024年3月29日 -- 2025年3月30日

已獲點(diǎn)擊:113

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B16-Blue™ IFN-γ cells do not respond to IFN-α/β, due to the inactivation of the type I IFN receptor.

Cell line description:

B16-Blue™ IFN-γ cells derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP (secreted embryonic alkaline phosphatase) reporter gene under the control of the IFN-inducible ISG54 promoter enhanced by a multimeric interferon-sensitive response element (ISRE).
B16-Blue™ IFN-γ cells do not respond to IFN-α/β, due to the inactivation of the type I IFN receptor. B16-Blue™ IFN-γ cells respond specifically to mIFN-γ and do not respond to human IFN-γ.

Stimulation of B16-Blue™ IFN-γ cells with mIFN-γ triggers the production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™ Solution, a medium that turns purple/blue in the presence of SEAP and by reading the OD at 655 nm.


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