中英文丨人干擾素Y ELISA開(kāi)發(fā)試劑盒說(shuō)明書(shū)

Analyte Information

IFN-γ

Interferon-γ (IFN-γ, also known as IFN-gamma, IFN-g, IFNg and Interferon-gamma) is produced mainly by activated T cells and NK cells. It is a proinflammatory cytokine that activates macrophages and endothelial cells, but it also regulates immune responses by effecting APC and T and B cells. Production of IFN-γ by helper T cells as well as cytotoxic T cells is a hallmark of the TH1-type phenotype, thus, high-level production of IFN-γ is typically associated with effective host defense against intracellular pathogens.

ELISABASIC kit for quantification of IFN-γ in solution

The human IFN-γ ELISABASIC kit is ideal for users who want the flexibility of setting up their own ELISA assay. This product enables quantification of human IFN-γ in solution, e.g. in cell culture supernatant. The kit contains a matched pair of monoclonal capture and detection antibodies, Streptavidin-HRP, ELISA standard and standard reconstitution buffer. Plates and substrate are not included.

Human IFN-γ ELISABASIC kits are also available with Streptavidin-ALP.

Serum/Plasma samples

Analysis of serum/plasma samples with this kit requires the use of ELISA diluent for dilution of the samples, standard and detection antibody. The diluent prevents false positive read-outs which may be caused by interference of heterophilic antibodies found in serum and plasma. The ELISA diluent is available from Mabtech. See Related products.

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艾美捷人干擾素Y ELISA開(kāi)發(fā)試劑盒，用于定量溶液中的 IFN-γ

人干擾素Y ELISA開(kāi)發(fā)試劑盒非常適合希望靈活設(shè)置自己的 ELISA 檢測(cè)的用戶。該產(chǎn)品可以對(duì)溶液中的人 IFN-γ 進(jìn)行定量，例如在細(xì)胞培養(yǎng)上清液中。該試劑盒包含一對(duì)匹配的單克隆捕獲和檢測(cè)抗體、Streptavidin-HRP、ELISA 標(biāo)準(zhǔn)和標(biāo)準(zhǔn)重組緩沖液。不包括板和基板。人干擾素Y ELISA開(kāi)發(fā)試劑盒也可與 Streptavidin-ALP 一起使用。

血清/血漿樣本

使用艾美捷人干擾素Y ELISA開(kāi)發(fā)試劑盒分析血清/血漿樣品，需要使用 ELISA 稀釋劑來(lái)稀釋樣品、標(biāo)準(zhǔn)品和檢測(cè)抗體。稀釋劑可防止由于血清和血漿中發(fā)現(xiàn)的異嗜性抗體的干擾而導(dǎo)致的假陽(yáng)性讀數(shù)。ELISA稀釋劑可從Mabtech獲得。僅供研究使用 (RUO)。不用于診斷過(guò)程。

艾美捷人干擾素Y ELISA開(kāi)發(fā)試劑盒標(biāo)準(zhǔn)曲線的繪制：

艾美捷人干擾素Y ELISA開(kāi)發(fā)試劑盒內(nèi)容：

捕獲 mAb (1-D1K)

生物素化檢測(cè) mAb (7-B6-1)

鏈霉親和素-HRP

重組人 IFN-γ ELISA 標(biāo)準(zhǔn)品

標(biāo)準(zhǔn)重組緩沖液

應(yīng)用：酶聯(lián)免疫吸附試驗(yàn)

反應(yīng)性：人類(lèi)

標(biāo)準(zhǔn)范圍：4-400 皮克/毫升