商品信息

產(chǎn)品英文名稱	Human IGF1 Protein
產(chǎn)品中文名稱	樣生長因子1（IGFI）
別名	Long R3 IGF-I; Insulin-like growth factor I; MGF; Somatomedin-C; IBP1

商品屬性

標(biāo)簽	無標(biāo)簽
表達(dá)宿主	大腸桿菌
種屬	人
序列	氨基酸序列來源于：樣生長因子1（IGFI) (P05019-1) (Gly49-Ala118) 表達(dá)的蛋白片段。
活性	通過其誘導(dǎo)MCF-7細(xì)胞增殖的能力來測定。該效應(yīng)的ED50為76.41 ng/mL。
蛋白長度	樣生長因子1（IGFI） 由71個氨基酸組成，預(yù)測分子量為7.8 KD。
純度	> 99% ，使用SDS-PAGE檢測
特點(diǎn)&優(yōu)勢	通過LAL法測定，每微克蛋白里內(nèi)毒素含量<1.0>
產(chǎn)品形式	凍干粉，凍干緩沖液為無菌的20 mM Tris，150 mM NaCl，pH 7.4。
基因ID	3479
蛋白序列鏈接	/uniprotkb/P05019/entry#P05019-1
使用中注意事項(xiàng)	開蓋使用前，請先離心。本產(chǎn)品為凍干粉，推薦用該產(chǎn)品配套的Reconstitution Buffer進(jìn)行復(fù)溶，且濃度不低于100 µg/mL。客戶可根據(jù)后續(xù)的實(shí)驗(yàn)需求，進(jìn)行進(jìn)一步的稀釋。
保存建議	本產(chǎn)品為凍干粉形式，建議：將凍干粉產(chǎn)品保存于-20℃以下的干燥環(huán)境中。本產(chǎn)品溶解之后，可在4℃保存2-7天，長期使用請置于-20℃以下。為長期保存，推薦添加載體蛋白（0.1% HAS/BSA或13%FBS），并酌情分裝保存，避免反復(fù)凍融。
運(yùn)輸條件	冰袋運(yùn)輸（藍(lán)冰）
警告	本文列出的產(chǎn)品僅供研究使用，不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用，不是建議使用我們的產(chǎn)品去違反任何或許可證。對于使用本產(chǎn)品可能發(fā)生的侵權(quán)或其他違規(guī)行為，我們不承擔(dān)任何責(zé)任。

附加信息

背景

IGF I, also known as Mechano Growth Factor, somatomedin-C, IGF-I, and IGF1, is a secreted protein that belongs to the insulin family. The insulin family, comprised of insulin, relaxin, insulin-like growth factors I and II ( IGF-I and IGF-II ), and possibly the beta-subunit of 7S nerve growth factor, represents a group of structurally related polypeptides whose biological functions have diverged. The IGFs, or somatomedins, constitute a class of polypeptides that have a key role in pre-adolescent mammalian growth. IGF-I expression is regulated by GH and mediates postnatal growth, while IGF-II appears to be induced by placental lactogen during prenatal development. IGF1 / IGF-I may be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. IGF1 / IGF-I stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also about enhancing glucose uptake. Defects in IGF1 / IGF-I are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) which is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness, and mental retardation.

圖片及說明