昊然生物-Alexa Fluor594-streptavidin，熒光AF594可以標(biāo)記蛋白/抗體

昊然生物-Alexa Fluor594-streptavidin，熒光AF594可以標(biāo)記蛋白/抗體

昊然生物分享熒光AF594可以標(biāo)記蛋白/抗體：

文獻(xiàn)描述：

Fluorescent dye alexa-fluor-594 was fixed to biotin with a spacer chain of four atoms. The static, dynamic fluorescence and fluorescence polarization properties of the avidin/labeled-biotin conjugate were investigated and compared with those of free fluorescent dye and directly labeled avidin. With 0.8 label on the average per avidin, one single fluorescence lifetime and one single correlation time related to the actual protein motion were recorded. Moreover, the excited state fluorescent probe is stabilized in the conjugate, with a quantum yield of 0.87, by slowing down the non-radiative processes, while fluorescence lifetime and fluorescence quantum yield increase by 10%, with respect to free alexa-fluor-594.

產(chǎn)品名稱：Alexa Fluor594-streptavidin

中文名稱：AF594標(biāo)記鏈霉親和素

英文名稱：Alexa Fluor594-streptavidin

分類：蛋白標(biāo)記

溶解性：/

規(guī)格：mg

純度：95%+

儲存：/

用途：科研

供貨商：西安昊然生物科技有限公司

Alexa Fluor 594可以通過生物SU-鏈霉親和素（Biotin-Streptavidin）相互作用來偶聯(lián)鏈霉親和素。這種方法通常用于將Alexa Fluor 594或其他熒光染料標(biāo)記到生物su修飾的分子或顆粒上，以進(jìn)行生物標(biāo)記、熒光顯微鏡成像或流式細(xì)胞儀分析等應(yīng)用。

可以使用激發(fā)適當(dāng)波長的光來激發(fā)Alexa Fluor 594或其他熒光染料，然后檢測并記錄熒光信號。這可以通過熒光顯微鏡、流式細(xì)胞儀或熒光分光光度計等設(shè)備來完成。

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西安昊然僅用于科研，RL2023.9