RGDSP功能化肽水凝膠，促進(jìn)人羊膜間充質(zhì)干細(xì)胞創(chuàng)面愈合

2024年10月，貴州省細(xì)胞工程重點(diǎn)實驗室，遵義醫(yī)學(xué)院附屬醫(yī)院,遵義醫(yī)科大學(xué)附屬醫(yī)院臨床干細(xì)胞研究所，湖北醫(yī)科大學(xué)附屬太和醫(yī)院創(chuàng)面修復(fù)與皮膚外科 (Key Laboratory of Cell Engineering of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Zunyi, China,The Clinical Stem Cell Research Institute, Affiliated Hospital of Zunyi Medical University, Zunyi, China,Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, China) Wei Wei老師研究團(tuán)隊在《Frontiers in Bioengineering and Biotechnology》上發(fā)表論文：

“RGDSP-functionalized peptide hydrogel stimulates growth factor secretion via integrin αv/PI3K/AKT axis for improved wound healing by human amniotic mesenchymal stem cells”

“RGDSP功能化肽水凝膠通過整合素αv/PI3K/AKT軸刺激生長因子分泌，促進(jìn)人羊膜間充質(zhì)干細(xì)胞創(chuàng)面愈合”

Abstract：

The wound healing process involves communication among growth factors, cytokines, signaling pathways, and cells in the extracellular matrix, with growth factors acting as key regulators. Although stem cells can promote wound healing by secreting diverse growth factors, their therapeutic potential is hindered by poor survival and engraftment. Mimicking the stem cell-matrix interactions can improve stem cell survival, regulate their fate, and even enhance their paracrine effects. This study investigated the use of composite RGDmix hydrogel, which can support the survival and proliferation of human amniotic mesenchymal stem cells (hAMSCs), and effectively increase the expression of various growth factors, thereby promoting wound re-epithelialization, angiogenesis, and epidermal maturation. At last, the specific role of integrin αv and PI3K/AKT signaling pathways in the secretion of growth factors were examined by silencing them in vitro and in vivo. Results suggested that the RGDmix hydrogel improved the secretion of growth factors by hAMSCs through the RGDSP/integrin αv/PI3K/AKT axis, thereby enhancing the therapeutic effect in wound healing.

摘要：

傷口愈合過程涉及生長因子、細(xì)胞因子、信號通路和細(xì)胞外基質(zhì)細(xì)胞之間的交流，其中生長因子是關(guān)鍵的調(diào)節(jié)因子。雖然干細(xì)胞可以通過分泌多種生長因子促進(jìn)傷口愈合，但其治療潛力受到生存和植入性差的阻礙。模擬干細(xì)胞與基質(zhì)的相互作用可以提高干細(xì)胞的存活率，調(diào)節(jié)其命運(yùn)，甚至增強(qiáng)其旁分泌作用。本研究研究了復(fù)合RGDmix水凝膠能夠支持人羊膜間充質(zhì)干細(xì)胞（hAMSCs）的存活和增殖，并有效增加各種生長因子的表達(dá)，從而促進(jìn)創(chuàng)面再上皮化、血管生成和表皮成熟。最后，通過體外和體內(nèi)實驗，研究整合素αv和PI3K/AKT信號通路在生長因子分泌中的具體作用。結(jié)果表明，RGDmix水凝膠通過RGDSP/integrin αv/PI3K/AKT軸促進(jìn)hAMSCs分泌生長因子，從而增強(qiáng)傷口愈合的治療效果。

該論文中，hAMSCs的體外培養(yǎng)是使用Ausbian特級胎牛血清完成的。