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90100ESRecombinant Human IL-1 alpha Protein
參考價(jià)535-5785
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  • 90100ES 型號(hào)
  • Yeasen/翌圣生物 品牌
  • 生產(chǎn)商 廠商性質(zhì)
  • 上海市 所在地

規(guī)格
2 μg535元99 件 可售
10 μg1855元99 件 可售
50 μg3855元99 件 可售

訪問(wèn)次數(shù):176更新時(shí)間:2024-05-09 09:22:20

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初級(jí)會(huì)員·13年
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曹女士
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Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1,and is a cytokine of the interleukin-1 family. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and h
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Product Description

Interleukin-1 alpha (IL1a) is also known as  IL-1A, IL1,and is a cytokine of the interleukin-1 family. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. IL-1 is a major immunoregulatory/proinflammatory cytokine which also affects fibroblast proliferation and function and therefore it was of interest to investigate whether its constitutive expression influences the in vivo tumorigenic potential of transformed fibroblastoid cell lines

 

Product Properties

Synonyms

Interleukin-1 alpha, IL-1α, BAF, IL-1F1, LAF, LEMpreinterleukin 1 alpha, pro-interleukin-1-alpha

Accession

 P01583

GeneID

3552

Source

E.coli-derived human IL-1alpha proteinSer113-Ala271.

Molecular Weight

Approximately 18.0 kDa.

AA Sequence

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Tag

None

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

> 97% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0 pg/mL, corresponding to a specific activity of > 1.0 × 109 IU/mg.

Endotoxin

< 1.0 EU per 1μg of the protein by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

Shipping and Storage

The products are shipped with ice pack and can be stored at -20 to -80 for 1 year.

Recommend to aliquot the protein into smaller quantities when first used and avoid repeated freeze-thaw cycles.

 

Cautions

  1. Avoid repeated freeze-thaw cycles.

  2. For your safety and health, please wear lab coats and disposable gloves for operation.

  3. For research use only.

HB220210





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