美國CYTOO細(xì)胞微納米結(jié)構(gòu)圖案多孔板和芯片與xonamicrofluidics品牌微流控細(xì)胞微圖案化培養(yǎng)和分化、相互作用資料

美國CYTOO細(xì)胞微納米結(jié)構(gòu)圖案多孔板和芯片應(yīng)用文獻(xiàn)
xonamicrofluidics品牌微流控細(xì)胞微圖案化產(chǎn)品應(yīng)用文獻(xiàn)

基底材料的拓?fù)湫蚊彩怯绊懠?xì)胞行為的重要因素之一,細(xì)胞與微納米結(jié)構(gòu)圖案表面相互作用研究在細(xì)胞生物學(xué)、再生醫(yī)學(xué)和組織工程領(lǐng)域具有重要的意義。材料表面微納米圖案化不但可以提供結(jié)構(gòu)模版,用以研究細(xì)胞對生長環(huán)境的響應(yīng)特性,而且可以為組織再生用支架和植入性器件的設(shè)計提供基礎(chǔ)數(shù)據(jù)。

實驗結(jié)果顯示,細(xì)胞會根據(jù)材料基底表面的圖案結(jié)構(gòu)產(chǎn)生相應(yīng)的形變,而且這種形變響應(yīng)與圖案幾何形狀、尺寸,細(xì)胞伸展和基底表面的黏附蛋白濃度有關(guān)。在一定范圍內(nèi),圖案越大、越深,細(xì)胞形變越大；細(xì)胞伸展面積越大,形變越?。吼じ降鞍卓梢源龠M(jìn)細(xì)胞伸展,從而使細(xì)胞形變減小.微米坑圖案能夠明顯促進(jìn)細(xì)胞在材料表面的黏附；微米溝槽圖案 則對細(xì)胞具有接觸誘導(dǎo)效應(yīng),可以誘導(dǎo)細(xì)胞響應(yīng)所生長的微納米溝槽結(jié)構(gòu)進(jìn)行骨架重排,從而引起細(xì)胞取向行為變化.

CYTOO品牌的細(xì)胞微圖案產(chǎn)品包括CYTOOPLATE?細(xì)胞微圖案微孔培養(yǎng)板和CYTOOChips細(xì)胞微圖案芯片:

CYTOOPLATE?細(xì)胞微圖案微孔培養(yǎng)板是一個高清晰度的低熒光硼硅玻璃底細(xì)胞光刻微圖案， CYTOOChips細(xì)胞微圖案芯片

可以控制細(xì)胞粘附性狀、大小、細(xì)胞培養(yǎng)分化等，研究細(xì)胞取，這種方法可以得到穩(wěn)定的具有顯著細(xì)胞黏附反差的 "微圖案化表面. 利用該圖案化表面技術(shù)引導(dǎo)細(xì)胞黏附后的取向，先在細(xì)胞無法貼附的Cytophobic 涂料處理，再以光蝕刻 "的技術(shù)，精準(zhǔn)地留下特定的幾何形狀，并在這樣的幾形狀上涂上特定的細(xì)胞間質(zhì)蛋白(extracellular " matrix , ECM, such as fibronecin, collgene, Laminin, ect…) 。這樣的細(xì)胞培養(yǎng)工具借由精準(zhǔn)的控制細(xì)胞貼附的型式，提供了體外培養(yǎng)細(xì)胞結(jié)構(gòu) 上的引導(dǎo)，使細(xì)胞表現(xiàn)出更接近體內(nèi)生理的表型及框架，這樣的工具可以:
◆提高試驗的再現(xiàn)性，靈敏性和歸納量化復(fù)雜的生物性狀
◆進(jìn)一步分析細(xì)胞結(jié)構(gòu)機械性刺激和細(xì)胞功能之間的關(guān)系
◆模擬重現(xiàn)細(xì)胞在生物體內(nèi)的環(huán)境，并使性狀易于觀察分析

"CYTOO 細(xì)胞微圖案(CYTOO micropattern)使體外培養(yǎng)細(xì)胞重現(xiàn)體內(nèi)應(yīng)有的細(xì)胞極性，有組織的整齊排列，細(xì)胞移動的限制!

CYTOO 細(xì)胞粘附微圖案

量化活細(xì)胞分析的突破
精準(zhǔn)的對照條件在CYTOO 陣列上，細(xì)胞呈現(xiàn)相同的結(jié)構(gòu)

標(biāo)準(zhǔn)的培養(yǎng)皿中的細(xì)胞 CYTOO培養(yǎng)產(chǎn)品中的細(xì)胞
"細(xì)胞貼附在涂有細(xì)胞間質(zhì)蛋白的微圖案(micropattern)陣列上時，會隨著微圖案（micropattern）的引導(dǎo)在不能貼附的材質(zhì)上張開成特定的幾何結(jié)構(gòu)。這樣特定形狀的貼附接觸分布，促使細(xì)胞產(chǎn)生固定且再現(xiàn)性*的極性。細(xì)胞位置、細(xì)胞形狀、細(xì)胞極性、以及胞器位置都可標(biāo)準(zhǔn)化.
重現(xiàn)體內(nèi)細(xì)胞的物理環(huán)境
使體外培養(yǎng)細(xì)胞的生理性狀更接近體內(nèi)組織

CYTOOchipTM細(xì)胞微圖案芯片
19.5x19.5 mm 蓋玻片，由光刻法在高質(zhì)低熒光硼硅酸鹽玻璃上刻畫微圖形，用于倒置顯微鏡， "網(wǎng)狀結(jié)構(gòu)打印在芯片底部，使用戶可以隨時找到所需細(xì)胞。這些芯片與CYTOOchamber芯片槽結(jié)合可實現(xiàn) 高分辨率活細(xì)胞成像。
標(biāo)準(zhǔn)芯片和平板可用于所有一般用途，初始包的設(shè)計是用來開發(fā)微圖形形狀和尺寸的， Arena芯片分為不同尺寸的磁盤，客戶定制產(chǎn)品可以使您達(dá)到*實驗方案。

CYTOOchips細(xì)胞微圖案芯片客戶定制產(chǎn)品包括19.5x19.5 mm 蓋玻片，客戶可定制微圖形，且粘附蛋白可適用于多種應(yīng)用和實驗。定制CYTOOchipszui小量為1組18個芯片，且包裹纖連蛋白或活性表面。
客戶定制范圍包括：

在我們的標(biāo)準(zhǔn)幾何圖形的基礎(chǔ)上，更大、更小、或密度不同
混合不同的微圖形形狀
調(diào)整芯片表面、面積或形狀，方便研究幾何圖形如何影響細(xì)胞結(jié)構(gòu)和行為
平行放置不同微圖形
zui低線寬3微米，點5微米

產(chǎn)品規(guī)格

CYTOOCHIPS細(xì)胞微圖案芯片,一包18個
產(chǎn)品名稱	貨號	(PATTERN)圖案	SIZE規(guī)格	COATING 涂層
CYTOOCHIPS? MOTILITY A X18	10-031-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? ARENA A X18	10-020-00-18	Disc	Multiple	Activated
CYTOOCHIPS? CUSTOM A X18	10-950-00-18	Multiple	Custom	Activated
CYTOOCHIPS? L-L-A X18	10-015-00-18	L	Large (1600 μm²)	Activated
CYTOOCHIPS? L-S-A X18	10-013-00-18	L	Small (700 μm²)	Activated
CYTOOCHIPS? L-M-A X18	10-014-00-18	L	Medium (1100 μm²)	Activated
CYTOOCHIPS? STARTER'S A X18	10-900-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? DC-S-A X18	10-001-00-18	Disc	Small (700 μm²)	Activated
CYTOOCHIPS? DC-M-A X18	10-002-00-18	Disc	Medium (1100 μm²)	Activated
CYTOOCHIPS? DC-L-A X18	10-003-00-18	Disc	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-L-A X18	10-006-00-18	Crossbow	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-S-A X18	10-004-00-18	Crossbow	Small (700 μm²)	Activated
CYTOOCHIPS? CW-M-A X18	10-005-00-18	Crossbow	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-L-A X18	10-012-00-18	Y	Large (1600 μm²)	Activated
CYTOOCHIPS? Y-M-A X18	10-011-00-18	Y	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-S-A X18	10-010-00-18	Y	Small (700 μm²)	Activated
CYTOOCHIPS? H-L-A X18	10-009-00-18	H	Large (1600 μm²)	Activated
CYTOOCHIPS? H-M-A X18	10-008-00-18	H	Medium (1100 μm²)	Activated
CYTOOCHIPS? H-S-A X18	10-007-00-18	H	Small (700 μm²)	Activated

CYTOOplates細(xì)胞微圖案培養(yǎng)板

CYTOOplates平板為標(biāo)準(zhǔn)SBS微平板版式，由光刻法在高質(zhì)低熒光硼硅酸鹽玻璃底部刻畫微圖形，可適用于高內(nèi)涵篩選，且可適用于所有普通HCS成像平臺和實驗室自動化儀器。
"CYTOOplates客戶定制產(chǎn)品包括96孔標(biāo)準(zhǔn)SBS版式，客戶可定制微圖形，且粘附蛋白可適用于多種應(yīng)用和實驗， "無論所需的微圖形形狀、尺寸或96孔布局如何。定制zui小量為10個平板，且包裹纖連蛋白或活性表面。
客戶定制范圍包括:

在我們的標(biāo)準(zhǔn)幾何圖形的基礎(chǔ)上，更大、更小、或密度不同
混合不同的微圖形形狀
調(diào)整平板表面、面積或形狀，方便研究幾何圖形如何影響細(xì)胞結(jié)構(gòu)和行為
平行放置不同微圖形
zui低線寬3微米，點5微米

CYTOOPLATES細(xì)胞微圖案培養(yǎng)板訂貨信息,一包5塊
產(chǎn)品名稱	貨號	(PATTERN)圖案	SIZE規(guī)格	COATING涂層
CYTOOPLATES? 96 RW L-S-A	20-013-00	L	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW L-L-A	20-015-00	L	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW L-M-A	20-015-00	L	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-S-A	20-001-00	Disc	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW DC-M-A	20-002-00	Disc	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-L-A	20-003-00	Disc	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW CW-S-A	20-004-00	Crossbow	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW CW-M-A	20-005-00	Crossbow	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW CW-L-A	20-006-00	Crossbow	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW H-S-A	20-007-00	H	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW H-M-A	20-008-00	H	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW H-L-A	20-009-00	H	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW Y-S-A	20-010-00	Y	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW Y-M-A	20-011-00	Y	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW Y-L-A	20-012-00	Y	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW STARTER	20-900-00	Multiple	Multiple	Activated
CYTOOPLATES? 96 RW MOTILITY A	20-031-00	Line	Multiple	Activated

CYTOO chamber芯片槽
單孔芯片槽提供了大面積的成像區(qū)域，可承裝10,000個細(xì)胞圖形，這款產(chǎn)品尤其適合當(dāng)您有超過4 "個不同的幾何圖形打印在芯片上的時候，以及像是有絲分裂的細(xì)胞的特例測量。次產(chǎn)品更適用于高分辨率活細(xì)胞成像。
4孔芯片槽可提供每孔上千種圖形在各情境下的平行免疫熒光實驗，Arena芯片和Motility芯片的4象限設(shè)計使之適用于4孔芯片槽。
優(yōu)勢和主要特征:

可重復(fù)使用，高溫滅菌
磁性裝置使用簡單
35mm足跡
與普通顯微鏡臺兼容
zui多四平行

CYTOO chamber灌流槽
"閉合單通道或雙通道CYTOOchamber灌流槽用于活細(xì)胞成像,使用簡單,專門設(shè)計用于我們的CYTOOchips芯片. 主要部分為透明的丙烯酸材料，底板是黑色的陽極化處理鋁合金，部件還包括一圈硅墊。通道尺寸可根據(jù)要求定制。
優(yōu)勢和主要特征：

- 閉合灌流槽
- 培養(yǎng)基補充簡易
- 與CYTOOchips兼容
- 磁性裝置使用簡單
- 可重復(fù)利用
- 單雙孔道標(biāo)準(zhǔn)配置，客戶定制通道尺寸
- 可配置顯微鏡臺適配器

Xonamicrofluidics品牌細(xì)胞微溝槽圖案流體培養(yǎng)板
貨號	產(chǎn)品名稱	圖片
RD150	Round Device (150 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 150 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom dishes can be used, as well as glass cover slips, if desired.
RD450	Round Device (450 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 450 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom "dishes can be used, as well as glass cover slips, if desired.
RD900	Round Device (900 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 900 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or "more to accommodate the device. At least 22 mm is recommended in order to "have room to position the device. Larger glass bottom dishes can be used, as "well as glass cover slips, if desired.
SND150	Standard Neuron Device (150 um microgroove barrier) Size: ~ 22 mm – ~23mm The 150 um neuron device with its shorter microgroove barrier is suitable for researchers who would like to isolate both axons and dendrites. It is also suitable for certain "neuronal cultures that do not grow long processes. Researchers who wish to study the early events of axonal and dendritic growth "may also find the 150 um device useful. The 150 um device "also offers the benefits of fluidic isolation and culture organization for transport studies.
SND450	Standard Neuron Device (450 um microgroove barrier) Size: ~ 22 mm – ~23mm "The 450 um neuron device is our most popular device. For most "applications it is suitable for separating cell bodies from axons. After two weeks, dendrites in E18 rat cortical neurons have typically not crossed the microgroove barrier. "The 450 um neuron device also provides the added features of fluidic "isolation and culture organization for transport studies.
SND900	Standard Neuron Device (900 um microgroove barrier) Size: ~ 22 mm – ~23mm The 900 um neuron device, with its wide microgroove barrier, is suitable for researchers who would like to perform long term experiments while ensuring axonal isolation. "In some cultures, after two weeks of growth dendrites may cross the 450 um barrier thereby necessitating the use of the 900 um device.It is also suitable "for certain neuronal cultures which grow long processes. Researchers who "also wish to make sure they are isolating * axons only in the distal "chamber may also find the 900 um device useful. The 900 um device also offers the benefits of fluidic isolation and culture organization for transport studies.
TCND500	Triple Chamber Neuron Device (500 um microgroove barrier) Size: ~ 38 mm – ~24mm The TCND500 consists of (2) 500 um microgroove barriers with a 500 um central chamber. This device makes it possible to culture cortical "neurons in the left and right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments applied to the left and right compartments.
TCND1000	Triple Chamber Neuron Device (1000 um microgroove barrier) Size: Size: ~ 38 mm – ~24mm The TCND1000 consists of (2) 500 um microgroove barriers with a 1000 um central chamber. This device makes "it possible to culture cortical neurons in the left and "right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments "applied to the left and right compartments.
uLP	Microfluidic Local Perfusion Device barrier, (50 um microgroove 75 micron perfusion channel, 500 um microgroove barrier) Size: ~ 40 mm – ~24 mm "Our Microfluidic Local Perfusion Chamber was first published in Neuron. "2010 Apr 15;66(1):57-68. This device has a 75 micron perfusion "chamber with a set of microgrooves on the left 50 um in width and a set of "microgrooves on the right 500 um in width. This device, in conjuction with a "syringe pump or other suitable equipment, allows the researcher to perfuse "selected treatments and compounds across the target area. Syringe pump or "other suitable equipment required for proper use. For more indepth "information please see Neuron. 2010 Apr 15;66(1):57-68.