CD23（B細(xì)胞）鼠單克隆抗體

CD23(B細(xì)胞)鼠單克隆抗體

廣州健侖生物科技有限公司

CD23 是一種分子量為 45KDa的細(xì)胞膜表面糖蛋白，是存在于 B細(xì)胞表面的低親和性 IgE 受體。表達(dá)于外周血細(xì)胞的某些亞群、 B淋巴細(xì)胞、淋巴濾泡樹突細(xì)胞(FDC)、初始淋巴細(xì)胞和 EB 病毒轉(zhuǎn)染的 B 淋巴瘤細(xì)胞系。慢性淋巴細(xì)胞白血病和小淋巴細(xì)胞性淋巴瘤瘤細(xì)胞也表達(dá)CD23，但套細(xì)胞淋巴瘤不表達(dá) CD23。

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞膜

克隆號(hào)：MRQ-57

同型：IgG1/K

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：扁桃體

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

CD23(B細(xì)胞)鼠單克隆抗體

測(cè)定微生物細(xì)胞數(shù)量的方法很多，通常采用的有顯微鏡直接計(jì)數(shù)法和稀釋平板計(jì)數(shù)法。
直接計(jì)數(shù)法適用于各種單細(xì)胞菌體的純培養(yǎng)懸浮液，如有雜菌或雜質(zhì)，則難于直接測(cè)定。菌體較大的酵母菌或霉菌孢子可采用血球計(jì)數(shù)板，一般細(xì)菌則采用彼德羅夫·霍澤（Petrof Hausser）細(xì)菌計(jì)數(shù)板。兩種計(jì)數(shù)板的原理和部件相同，只是細(xì)菌計(jì)數(shù)板較薄，可以使用油鏡觀察。而血球計(jì)數(shù)板較厚，不能使用油鏡，計(jì)數(shù)板下部的細(xì)菌難于區(qū)分。
血球計(jì)數(shù)板是一塊特制的厚型載玻片，載坡片上有4條槽所構(gòu)成的3個(gè)平臺(tái)。中間的平臺(tái)較寬，其中間又被一短橫槽分隔成兩半，每個(gè)半邊上面各有一個(gè)計(jì)數(shù)區(qū)（圖示），計(jì)數(shù)區(qū)的刻度有兩種：一種是計(jì)數(shù)區(qū)分為16個(gè)大方格，大方格用三線隔開，而每個(gè)大方格又分成25個(gè)小方格；另一種是一個(gè)計(jì)數(shù)區(qū)分成25個(gè)大方格，大方格之間用雙線分開，而每個(gè)大方格又分成16個(gè)小方格。但是不管計(jì)數(shù)區(qū)是哪一種構(gòu)造，它們都有一個(gè)共同特點(diǎn)，即計(jì)數(shù)區(qū)都由400個(gè)小方格組成（圖示）。
計(jì)數(shù)區(qū)邊長(zhǎng)為1mm，則計(jì)數(shù)區(qū)的面積為1mm2，每個(gè)小方格的面積為1/400 mm2。蓋上蓋玻片后，         
計(jì)數(shù)區(qū)的高度為0.1mm，所以計(jì)數(shù)區(qū)的體積為0.1mm3，每個(gè)小方格的體積為1/4000 mm3。
使用血球計(jì)數(shù)板計(jì)數(shù)時(shí)，先要測(cè)定每個(gè)小方格中微生物的數(shù)量，再換算成每mL菌液（或每g樣品）中微生物細(xì)胞的數(shù)量。
已知：1mL體積＝10 mm×10 mm×10 mm＝1000mm3       
所以：1mL體職應(yīng)含有小方格數(shù)為1000mm3/（1/4000mm3）＝4×106個(gè)小方格，即系數(shù)K＝4×106。
因此：每mL菌懸液中含有細(xì)胞數(shù)＝每個(gè)小格中細(xì)胞平均數(shù)（N）×系數(shù)（K）×菌液稀釋倍數(shù)（d）。
六、實(shí)驗(yàn)材料
活材料：釀酒酵母（Saccharomyces cerevisiae）斜面菌種或培養(yǎng)液、枯草桿菌（Bacillus subtilis）染色標(biāo)本片。

CD23(B細(xì)胞)鼠單克隆抗體

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

想了解更多的產(chǎn)品及服務(wù)請(qǐng)掃描下方二維碼：

【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

There are many ways to determine the number of microbial cells, usually the direct counting microscope and the dilution plate counting method.
Direct counting method for a variety of single cell suspension of pure culture, such as bacteria or impurities, it is difficult to direct determination. Larger cell strains of yeast or mold spores can be used blood count plate, the general bacteria are used Petrof Hausser bacteria count plate. The principle and components of the two counting plate the same, but the bacterial count plate is thin, you can use oil mirror observation. The blood count plate thicker, can not use the oil mirror, the lower part of the counting plate difficult to distinguish bacteria.
The blood cell counting plate is a special thick glass slide, which contains 3 platforms formed by 4 slots. The middle of the platform wider, the middle was a short groove separated by two halves, each half of each have a counting zone (Figure), there are two counting zone scale: a count is divided into 16 large Checkered, large squares separated by three lines, and each large square is divided into 25 small squares; the other is a count is divided into 25 large square, large squares separated by two lines, and Each big square is divided into 16 small squares. But regardless of the construction of the counting zone, they all have one thing in common: the counting zone consists of 400 small squares (pictured).
Counting area edge length is 1mm, then counting area area of ??1mm2, each small square area of ??1 / 400mm2. After covering the cover glass,
The height of the counting zone is 0.1 mm, so the volume of the counting zone is 0.1 mm3 and the volume of each small square is 1/4000 mm3.
When counting with a hemocytometer, the number of microbes in each small square is determined and then converted into the number of microbial cells per mL of broth (or per gram of sample).
It is known that 1 mL volume = 10 mm × 10 mm × 10 mm = 1000 mm 3
So: 1mL bodywork should contain a small number of squares 1000mm3 / (1 / 4000mm3) = 4 × 106 small squares, the coefficient K = 4 × 106.
Therefore: Number of cells contained in each mL of bacterial suspension = average number of cells in each cell (N) x coefficient (K) x bacterial dilution (d).
Six, experimental materials
Live material: Saccharomyces cerevisiae benthic strain or culture, Bacillus subtilis stained specimen.