ALK/p80間變性淋巴瘤激酶（鼠單克隆抗體）

5A4

廣州健侖生物科技有限公司

間變性大細(xì)胞淋巴瘤，即是非霍奇金淋巴瘤的一種獨(dú)立類型，由德國(guó)病理學(xué)家Stein等于1985年應(yīng)用Ki-1（CD30）抗體識(shí)別，常呈間變性特征，被命名為間變性大細(xì)胞淋巴瘤。REAL分類將B細(xì)胞表型者歸為彌漫性大B細(xì)胞性淋巴瘤。目前，ALCL只包括T表型和Null（非T非B）表型。約60%-85%左右ALCL病例表達(dá)間變性淋巴瘤激酶（anaplasticlymphomakinase，ALK）融合蛋白，這是由于2號(hào)染色體上的ALK基因位點(diǎn)的畸變所致。zui常見的是t（2；5）（p23；q35）而形成融合基因NPM-ALK，它是由位于5號(hào)染色體上的核仁磷酸蛋白B23（NPM）基因與位于2號(hào)染色體的ALK基因相融合形成，表達(dá)融合蛋白為NPM-ALK蛋白；zui近尚有更多的ALK基因與其他基因通過染色體轉(zhuǎn)位或者是染色體的倒轉(zhuǎn)而形成的融合基因被發(fā)現(xiàn)，如t（1；2）（q25；p23）所形成的TPM3-ALK基因，t（2；3）（p23；q21）產(chǎn)生的TFG-ALKs基因，TFG-ALKL基因和TFG-ALKxL基因，inv（2）（p23；q35）所形成的ATIC-ALK基因，t（2；17）（p23；q23）形成的CLTCL-ALK基因及t（X；2）（q11；p23）形成的-ALK基因。

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【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿/細(xì)胞核

克隆號(hào)：5A4

同型：IgG1

適用組織：石蠟/冰凍

陽性對(duì)照：間變性大細(xì)胞淋巴瘤/肺腺瘤

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：60min

ALK/p80間變性淋巴瘤激酶（鼠單克隆抗體）5A4

檢查
1.病原學(xué)檢查
（1）糞便檢查 ①活滋養(yǎng)體檢查法 常用生理鹽水直接涂片法檢查活動(dòng)的滋養(yǎng)體。急性痢疾患者的膿血便或阿米巴炎病人的稀便，要求容器干凈，糞樣新鮮、送檢越快、越好，寒冷季節(jié)還要注意運(yùn)送和檢查時(shí)的保溫。典型的阿米巴痢疾糞便為醬紅色黏液樣，有特殊的腥臭味。鏡檢可見黏液中含較多粘集成團(tuán)的紅細(xì)胞和較少的白細(xì)胞，有時(shí)可見夏科-雷登氏結(jié)晶和活動(dòng)的滋養(yǎng)體。這些特點(diǎn)可與細(xì)菌性痢疾的糞便相區(qū)別。②包囊檢查法 以竹簽沾取少量糞樣，在碘液中涂成薄片加蓋玻片，然后置于顯微鏡下檢查，鑒別細(xì)胞核的特征和數(shù)目。
（2）阿米巴培養(yǎng) 由于技術(shù)操作復(fù)雜，需一定設(shè)備，且阿米巴人工培養(yǎng)在多數(shù)亞急性或慢性病例陽性率不高，似不宜作阿米巴診斷的常規(guī)檢查。
（3）組織檢查 通過乙狀結(jié)腸鏡或纖維結(jié)腸鏡直接觀察黏膜潰瘍，并作組織活檢或刮拭物涂片，檢出率zui高。滋養(yǎng)體的取材必須在潰瘍的邊緣，鉗取后以局部稍見出血為宜。膿腔穿刺液檢查除注意性特征外，應(yīng)取材于膿腔壁部，較易發(fā)現(xiàn)滋養(yǎng)體。
2.免疫檢查
近年來國(guó)內(nèi)外陸續(xù)報(bào)告了多種血清學(xué)診斷方法，其中以間接血凝（IHA）、間接熒光抗體（IFAT）和酶聯(lián)免疫吸附試驗(yàn)（ELISA）研究較多，但敏感性對(duì)各型病例不同。IHA的敏感較高，對(duì)腸阿米巴病的陽性率達(dá)98%，腸外阿米巴病的陽性率達(dá)95%，而無癥狀的帶蟲者僅10%～40%，IFA敏感度稍遜于IHA。EALSA敏感性強(qiáng)，特異性高，有發(fā)展前途。近年來，已有報(bào)道應(yīng)用敏感的免疫學(xué)技術(shù)在糞便及膿液中檢測(cè)阿米巴特異性抗原獲得成功。特別是抗阿米巴雜音瘤單克隆抗體的應(yīng)用為免疫學(xué)技術(shù)探測(cè)宿主排泄物中病原物質(zhì)了可靠、靈敏和抗干擾的示蹤式具。
診斷
對(duì)阿米巴病的診斷，除根據(jù)患者的主訴、病史和臨床表現(xiàn)作為診斷依據(jù)外，重要的是病原學(xué)診斷，糞便中檢查到阿米巴病原體為惟一可靠的診斷依據(jù)。通常以查到大滋養(yǎng)體者作為現(xiàn)癥患者，而查到小滋養(yǎng)體或包囊者只作為感染者。
鑒別診斷
阿米巴腸病需和細(xì)菌性痢疾、血吸蟲病、腸結(jié)核、結(jié)腸癌、慢性非特異性潰瘍性結(jié)腸炎等鑒別。
1.細(xì)胞性痢疾
起病急，全身中毒癥狀嚴(yán)重，抗生素治療有效，糞便鏡檢和細(xì)菌培養(yǎng)有助于于診斷。
2.血吸蟲病
起病較緩，病程長(zhǎng)，有疫水接觸史，肝脾腫大，血中嗜酸粒細(xì)胞增多，糞便中可發(fā)現(xiàn)血吸蟲卵或孵化出毛蚴，腸黏膜活組織中可查到蟲卵。
3.腸結(jié)核
大多有原發(fā)結(jié)核病灶存在，患者有消耗性熱、盜汗、營(yíng)養(yǎng)障礙等；糞便多呈黃色稀粥狀，帶黏液而少膿血，腹瀉與便秘交替出現(xiàn)。胃腸道X線檢查有助于診斷。
4.結(jié)腸癌
患者年齡較大，多有排便習(xí)慣的改變，大便變細(xì)，有進(jìn)行性貧血，消瘦。晚期大多可捫及腹塊，X線鋇劑灌腸檢查和纖維結(jié)腸鏡檢查有助于診斷。
5.慢性非特異性潰瘍性結(jié)腸炎
臨床癥狀與慢性阿米巴病不易區(qū)別，但大便檢查不能發(fā)現(xiàn)阿米巴，且經(jīng)抗阿米巴治療仍不見效時(shí)可考慮本病。

ALK/p80間變性淋巴瘤激酶（鼠單克隆抗體）5A4

我司還提供其它進(jìn)口或國(guó)產(chǎn)試劑盒：登革熱、瘧疾、流感、A鏈球菌、合胞病毒、腮病毒、乙腦、寨卡、黃熱病、基孔肯雅熱、克錐蟲病、違禁品濫用、肺炎球菌、軍團(tuán)菌、化妝品檢測(cè)、食品安全檢測(cè)等試劑盒以及日本生研細(xì)菌分型診斷血清、德國(guó)SiFin診斷血清、丹麥SSI診斷血清等產(chǎn)品。

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】    楊永漢

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

an examination
1. Etiological examination
(1) stool examination ① live trophozoite test commonly used saline direct smear activity of trophozoites. Patients with acute dysentery or pus and blood in patients with amebiasis loose stools, requiring containers clean, fecal samples, inspection faster, the better, the cold season but also pay attention to transport and inspection of the insulation. Typical amebic dysentery faeces are reddish mucus-like, with a special stench. Microscopic examination showed mucus with more sticky into the group of red blood cells and less white blood cells, and sometimes see Xiake - Radom crystallization and activity of trophozoites. These characteristics can be distinguished from the diarrhea of ??bacterial diarrhea. ② cystic examination to take a small amount of bamboo stick stool sample, coated with iodine in a thin sheet coated glass, and then placed under a microscope to identify and identify the characteristics and number of nuclei.
(2) Amoeba culture due to technical operation is complicated, need certain equipment, and artificial c*tion of amoeba in the majority of subacute or chronic cases positive rate is not high, it may not be routine examination for amoebic diagnosis.
(3) histological examination by sigmoidoscopy or colonoscopy direct observation of mucosal ulcers, and for biopsy or wipe smear, the highest detection rate. Trophoblast drawing must be at the edge of the ulcer, after the clamp to take some bleeding is appropriate. Abscess puncture fluid examination in addition to note the characteristics, should be taken from the abscess wall, easier to find trophozoites.
Immunization
In recent years, many serological diagnostic methods have been reported both at home and abroad, including indirect hemagglutination (IHA), indirect immunofluorescence (IFAT) and enzyme-linked immunosorbent assay (ELISA), but the sensitivity of different types of cases . The sensitivity of IHA was higher, the positive rate of enteromycosis was 98%, the rate of extrahepatic amoebiasis was 95%, while that of asymptomatic insects was only 10% -40%. IFA sensitivity was slightly Less than IHA. EALSA is sensitive, specific and promising. In recent years, it has been reported that the detection of amebic antigen in stool and pus using a sensitive immunological technique has been reported successfully. In particular, the use of monoclonal antibodies against amoebiasis is a reliable, sensitive and anti-interference tracer for the detection of pathogenic agents in host excretions by immunological techniques.
diagnosis
The diagnosis of amebiasis, in addition to based on the patient's chief complaint, history and clinical manifestations as the basis for the diagnosis, the most important is the etiological diagnosis, detection of amebiasis in the stool is the only reliable basis for the diagnosis. Usually found to nourish people who are now as patients, and found that nodules or cysts only as infected.
Differential diagnosis
Amoeba enteropathy and bacterial diarrhea, schistosomiasis, intestinal tuberculosis, colon cancer, chronic nonspecific ulcerative colitis and other identification.
1. dysentery
Urgency, systemic symptoms of severe poisoning, antibiotic treatment is effective, stool microscopy and bacterial culture contribute to the diagnosis.
Schistosomiasis
Slow onset, long course of disease, a history of exposure to water, hepatosplenomegaly, blood eosinophilia, schistosomiasis eggs can be found in feces or hatching of cercariae, intestinal mucosa can be found in the living tissue eggs.
3. Intestinal tuberculosis
Most of the primary tuberculosis exists, the patient has a consumption of heat, night sweats, nutritional disorders, etc .; stools were mostly yellow gruel, with mucus and less pus and blood, diarrhea and constipation alternay. Gastrointestinal X-ray examination can help diagnose.
Colon cancer
Patients older, more changes in bowel habits, thinning stools, progressive anemia, weight loss. Late palpable abdominal mass, barium enema X-ray examination and colonoscopy help to diagnose.
5. Chronic nonspecific ulcerative colitis
Clinical symptoms and chronic amebiasis is not easy to distinguish, but stool examination can not be found amoeba, and anti-amoeba treatment is still not effective when the disease can be considered.