Cytokeratin HMW(高分子量細(xì)胞角蛋白)

廣州健侖生物科技有限公司

高分子量角蛋白抗體（AE3）能識(shí)別角蛋白1、5、10和14。該抗體能和鱗癌、腺鱗癌發(fā)生反應(yīng)，但不能識(shí)別腺癌。在正常組織中，AE3可顯示復(fù)層上皮、肌上皮細(xì)胞以及前列腺和支氣管的基底層細(xì)胞。AE3對(duì)于腺癌和鱗癌以及前列腺良惡性腫瘤的研究是非常有用的。

標(biāo)本來(lái)源 囑患者先行用清水漱口，指導(dǎo)其深咳出痰液置于無(wú)菌痰盒中立即送檢，氣管插管或氣管切開的患者，則以無(wú)菌吸痰管從氣管內(nèi)吸痰，低倍鏡視野下多核白細(xì)胞>25，上皮細(xì)胞<10個(gè)的痰標(biāo)本為合格標(biāo)本。
細(xì)菌培養(yǎng) 將處理后的痰標(biāo)本接種于血瓊脂培養(yǎng)基培養(yǎng)，18～24h后觀察菌落特征并染色。
藥物敏感性測(cè)定 多優(yōu)勢(shì)菌進(jìn)行分離鈍化和鑒定。菌種鑒定采用美國(guó)VITEK-32自動(dòng)微生物分析儀。用K-B紙片法對(duì)所分析的菌株進(jìn)行常用藥物的敏感性測(cè)定，質(zhì)控菌按衛(wèi)生部統(tǒng)一標(biāo)準(zhǔn)。嚴(yán)格按美國(guó)國(guó)家臨床實(shí)驗(yàn)委員會(huì)標(biāo)準(zhǔn)進(jìn)行操作和結(jié)果判斷，同時(shí)對(duì)革蘭陰性菌進(jìn)行ESBLs檢測(cè)。
結(jié)果
分離出127株細(xì)菌中，其中革蘭陰性菌95株，占74.8%，以銅綠假單胞菌，肺炎克雷伯菌和鮑曼不動(dòng)桿菌占的比例zui多，培養(yǎng)出兩種菌株以上的則有42例，混合感染以兩種革蘭陰性菌為主，其次為革蘭陰性菌合并真菌感染，產(chǎn)ES-BLs菌株則共17例，占17.9%。

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Cytokeratin HMW(高分子量細(xì)胞角蛋白)

【產(chǎn)品介紹】

細(xì)胞定位：細(xì)胞漿

克隆號(hào)：AE3

同型：IgG1

適用組織：石蠟/冰凍

陽(yáng)性對(duì)照：食道鱗癌

抗原修復(fù)：熱修復(fù)（EDTA）

抗體孵育時(shí)間：30-60min

Cytokeratin HMW(高分子量細(xì)胞角蛋白)

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【公司名稱】 廣州健侖生物科技有限公司
【市場(chǎng)部】     歐

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【騰訊  】 
【公司地址】 廣州清華科技園創(chuàng)新基地番禺石樓鎮(zhèn)創(chuàng)啟路63號(hào)二期2幢101-103室

High molecular weight keratin antibodies (AE3) recognize keratins 1, 5, 10 and 14. The antibody and squamous cell carcinoma, adenosquamous carcinoma reaction, but can not identify adenocarcinoma. In normal tissues AE3 can display stratified epithelium, myoepithelial cells, and basal cells of the prostate and bronchus. AE3 for adenocarcinoma and squamous cell carcinoma and prostate benign and malignant tumor research is very useful.

Origin of the specimen Zhu Huanzhe patients first mouthwash to guide their cough sputum immediay placed in a sterile sputum boxes, tracheal intubation or tracheotomy patients, the sterile suction tube from the endotracheal suction , Low magnification view of multicellular leukocytes> 25, epithelial cells <10 sputum specimens for qualified specimens.
Bacterial culture The treated sputum samples were inoculated on blood agar medium and the colony characteristics were observed and stained after 18 to 24 hours.
Drug susceptibility determination of multi-dominant bacteria for separation and passivation and identification. Strain identification using the United States VITEK-32 automatic microbial analyzer. Using K-B disc method for the analysis of the susceptibility of commonly used drugs to determine strains, quality control bacteria by the Ministry of Health uniform standards. In strict accordance with the standards of the National Committee for Clinical Trials and the results of the operation to determine the same time, Gram-negative bacteria ESBLs detection.
result
Of the 127 strains isolated, 95 strains of Gram-negative bacteria accounted for 74.8% of the total, accounting for the largest proportion of Pseudomonas aeruginosa, Klebsiella pneumoniae and Acinetobacter baumannii, There were 42 cases of mixed infection with two Gram-negative bacteria, followed by Gram-negative bacteria combined with fungal infection, ES-BLs strains produced a total of 17 cases, accounting for 17.9%.