TRAF6 Intrachain TR-FRET Assay Kit 返回列表頁

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產(chǎn)品型號78598

品牌BPS Bioscience

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Ubiquitination

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●Purchase●DescriptionTheTRAF6intrachainTR-FRETAssayKitisasensitivehigh-throughputscreening(HTS)TR-FRETAssayKit

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Description

The TRAF6 intrachain TR-FRET Assay Kit is a sensitive high-throughput screening (HTS) TR-FRET Assay Kit, designed to measure TRAF6 auto-ubiquitination activity in a homogeneous 384 reaction format. It utilizes a Europium-labeled ubiquitin (Ub) donor as well as Cy5-labeled Ub acceptor to complete the TR-FRET pairing. Since both the TR-FRET donor and acceptor are incorporated into poly-ubiquitin chains formed on TRAF6, this assay measures poly-ubiquitination. As a homogeneous assay, it requires no time-consuming washing steps, making it especially suitable for HTS applications as well as real-time analyses.

Figure 1. E3 ligase TRAF6 intrachain TR-FRET Assay Kit schematic.

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Synonyms

TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, Interleukin-1 signal transducer, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6

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